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Figure 4 | Molecular Cancer

Figure 4

From: Targeting Hsp90 with small molecule inhibitors induces the over-expression of the anti-apoptotic molecule, survivin, in human A549, HONE-1 and HT-29 cancer cells

Figure 4

17-AAG treatment induces over-expression of survivin through transcription-independent mechanisms. (A) Inhibition of Hsp90 reduced the amount of survivin mRNA transcript presented in 17-AAG treated A549 cells. Human A549 cancer cells were incubated with DMSO (negative control) or various concentrations (10 nM, 100 nM, 250 nM and 500 nM) of 17-AAG and geldanamycin for 24 hours. Quantitative RT-PCR was performed to determine the amount of survivin transcripts presented in cells. (B) Cells treated with 17-AAG and geldanamycin did not induce abnormal cell morphology. A549 cells incubated with various concentrations (250 nM and 500 nM) of 17-AAG and geldanamycin for 24 hours and cell morphology was revealed by phase-contrast microscopy. (C) Inhibition of Hsp90 increased the amount of survivin mRNA transcript presented in 17-AAG and geldanamycin treated HT-29 cells. HT-29 cells were incubated with DMSO (negative control) or various concentrations (10 nM, 100 nM, 250 nM and 500 nM) of 17-AAG for 24 hours. Quantitative RT-PCR was performed to determine the amount of survivin transcripts presented in cells. (D) Inhibition of Hsp90 increased the amount of survivin mRNA transcript presented in 17-AAG treated HONE-1 cells. HONE-1 cells were incubated with DMSO (negative control) or various concentrations (10 nM, 100 nM, 250 nM and 500 nM) of 17-AAG for 24 hours. Quantitative RT-PCR was performed to determine the amount of survivin transcripts presented in cells.

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