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Figure 7 | Molecular Cancer

Figure 7

From: Targeting Hsp90 with small molecule inhibitors induces the over-expression of the anti-apoptotic molecule, survivin, in human A549, HONE-1 and HT-29 cancer cells

Figure 7

Inhibition of Hsp90 with 17-AAG reduced proteasome activity in HONE-1 and HT-29 cells. (A) Cells were treated with various concentrations of 17-AAG for 24 h. Cells were washed with PBS and lysed with TNESV buffer without protease inhibitor. Cell lysate were analyzed for proteasome activity using synthetic fluorogenic peptide succinyl-Leu-Leu-Val-Tyr-7-amino-4-methylcoumarin. Statistical significance (p < 0.05) between experimental samples and the DMSO control is denoted by "*". (B) Inhibition of 26S proteasome further increased the amount of survivin protein in 17-AAG treated HONE-1 and HT-29 cells. Cells were treated with DMSO and various concentrations (100 nM, 250 nM, 500 nM) of 17-AAG with/without MG-132 for 24 hours. The expression of survivin was revealed by Western blot analysis. Actin was used as an internal control.

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Molecular Cancer

ISSN: 1476-4598