Reduced expression of EIF4G1 inhibited cell proliferation. A. Western blotting assay shows significantly decreased protein expression of EIF4G1 in shRNA-EIF4G1 cells comparing to shRNA-Ctr and the parental 5-8F cells. β-actin was used as the internal control. B. The cell growth of parental 5-8F cells and their stable derivatives, shRNA-Ctrl and shRNA-EIF4G1, were examined by MTT assay over a seven-day period. *P < 0.05, as compared to 5-8F and shRNA-Ctrl cells.