Reduced EIF4G1 expression inhibited cell migration, invasion and cell cycle progression. A. Cell cycle was determined by FACS Caliber Cytometry. Cell migration (B) and invasion (C) capabilities of parental 5-8F cells and their stable derivatives, shRNA-Ctrl and shRNA-EIF4G1, were examined using transwell assay. Data were presented as mean ± SD for three independent experiments. *P < 0.05, as compared to shRNA-Ctrl and 5-8F cells.