Reduced EIF4G1 expression inhibited cell colony formation and xenograft tumor growth in vivo. A. The cell clone ability of parental 5-8F cells and their stable derivatives, shRNA-Ctrl and shRNA-EIF4G1, were examined by plate colony formation assay. *P < 0.05, as compared to 5-8F and shRNA-Ctrl cells. B. 5-8F cells containing shRNA-Ctrl or shRNA-EIF4G1 lentiviral vectors were injected into nude mice (n = 6 for each group). After 25 d, tumors were harvested and weighted. Data are presented as mean ± SD. *P < 0.05 as compared to shRNA-EIF4G1 cells. C. EIF4G1 protein expression was markedly decreased in xenograft tumors induced by shRNA-EIF4G1 cells comparing to that induced by shRNA-Ctrl cells.