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Figure 2 | Molecular Cancer

Figure 2

From: Differential requirement of the epidermal growth factor receptor for G protein-mediated activation of transcription factors by lysophosphatidic acid

Figure 2

Modest increase in tyrosine phosphorylation of EGFR induced by LPA. A. Caov-3, Dov-13 and SKOV-3 cells were staved and stimulated for 30 min or otherwise indicated (hr) with LPA (10 μM), S1P (1 μM), LPA (10 μM)+S1P (1 μM) IGF (50 ng/ml), insulin (0.2 μM), PDGF (BB isoform, 50 ng/ml), EGF (25 ng/ml) or FBS (5%). Tyrosine phosphorylation of EGFR was examined by immunoblotting with an anti EGFR phospho-specific antibody that recognizes EGFR-p at Y1068. The membrane was reprobed for total EGFR or β actin. In the bottom panel, Caov-3 and OVCAR-3 cells were treated with LPA (10 μM) for the indicated periods of time (min) or with EGF (25 ng/ml) for 30 min as a positive control. EGFR was immunoprecipitated from cell lysates and then analyzed by immunoblotting with an anti phosphotyrosine antibody for tyrosine phosphorylation (Y-p). B. Caov-3 cells were treated with LPA or different doses of EGF as indicated. Induction of AP-1 proteins and EGFR-p was examined by immunoblotting.

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Molecular Cancer

ISSN: 1476-4598