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Figure 5 | Molecular Cancer

Figure 5

From: Differential requirement of the epidermal growth factor receptor for G protein-mediated activation of transcription factors by lysophosphatidic acid

Figure 5

Involvement of different G protein signaling cascades in LPA-induced activation of AP-1 and NF-κB. A. Inhibition of Gi with PTX attenuated AP-1 but not NF-κB activation by LPA. Caov-3 cells were starved and stimulated for the indicated periods of time with LPA (10 μM) in the presence or absence of PTX (25 ng/ml). PTX was added 6 hours before stimulation with LPA. LPA-induced phosphorylation of NF-κB p65 at Ser 536 and expression of c-Jun and c-Fos were analyzed by immunoblotting. B. Caov-3 cells were transfected using Amaxa with the Gq G208A dominant negative mutant (Gq-DN) or control vector. The transfected cells were starved and stimulated for 2 hr with LPA (10 μM) before immunoblotting for p65 phosphorylation and c-Jun and c-Fos expression. C. Caov-3 cells were starved and stimulated for indicated periods of time with LPA (10 μM) in the presence or absence of U73122 (10 μM) or Y27632 (7.5 μM) which were added 45 min before stimulation with LPA. NF-κB p65 phosphorylation and c-Jun and c-Fos expression were assessed by immunoblotting. Reprobing with tubulin or β actin was included as loading controls.

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Molecular Cancer

ISSN: 1476-4598