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Figure 6 | Molecular Cancer

Figure 6

From: Differential requirement of the epidermal growth factor receptor for G protein-mediated activation of transcription factors by lysophosphatidic acid

Figure 6

Differential requirement of EGFR for activation of G protein signaling cascades by LPA. A. Inhibition of EGFR blocked LPA-stimulated Ras but not Rho activation. Caov-3 cells were starved and stimulated for the indicated periods of time with LPA (5 μM) in the presence of AG1478 (1 μM) or vehicle (DMSO). The levels of Ras-GTP and Rho-GTP were determined by pulldown with GST-Raf and GST-Rhotekin, respectively as described in Methods. B. Inhibition of EGFR did not affect signaling of the Gq-PKC pathway. Caov-3 cells were starved and stimulated for the indicated periods of time with LPA (10 μM) in the presence of AG1478 (1 μM), GF109203X (2.5 μM) or vehicle. LPA-induced phosphorylation of PKD at Ser 916 and NF-κB p65 at Ser 536 was analyzed by immunoblotting followed by reprobing with β actin.

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Molecular Cancer

ISSN: 1476-4598