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Figure 4 | Molecular Cancer

Figure 4

From: Quantitative proteomics identification of phosphoglycerate mutase 1 as a novel therapeutic target in hepatocellular carcinoma

Figure 4

Suppression of PGAM1 by PGAM1-shRNA-a resulted in cell growth arrest and induced apparent apoptosis in vitro. (A) Evaluation of the antiproliferation effects using MTT assay. Inhibition of cell growth by siRNA against PGAM1 was in a duration-dependent manner and the proliferation rate was decreased to 48.6% at 72 h posttransfection. (B) Survival rates of hepatoma cells were examined by colony formation assay. 24 h after transfection, cells were allowed to grow at a density of 300 cells/dish for additional two weeks before staining with Crystal Violet. (C) Flow cytometry analysis was performed and the subdiploid peak increased after 72 h of transfection (p < 0.05). (D) Cell apoptosis was assessed by TUNEL assay which showed a remarkably greater percentage of TUMEL positive nuclei of the PGAM1-shRNA-a group vs. the control group. a, untreated; b, Lipofectamine 2000; c, shNC; d, PGAM1-shRNA-a. Results represent the average of three independent experiments and data were shown as mean ± S.D.

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