Figure 2

MCP-1 induction by TNF-α after reconstitution of wild-type p53 activities. 4Bv (A) and BT2E (B) cells were cultivated at 37°C or shifted to 32°C overnight. Stimulation was done with TNF-α for 4 h or 6 h. Total cellular RNA was separated in a 1% agarose gel and transferred to a Gene screen Plus membrane. The filter was subsequently hybridized with a p21, MCP-1 and c-myc cDNA probe. The positions of the 28S and 18S ribosomal RNA are indicated.