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Figure 5 | Molecular Cancer

Figure 5

From: MicroRNA-184 inhibits neuroblastoma cell survival through targeting the serine/threonine kinase AKT2

Figure 5

(A) Growth curves following transfection with each AKT2 siRNA and negative control for (A) Kelly (p values for each siRNA ranged from 0.01 to 0.03) and (B) SK-N-AS (p values for each siRNA ranged from 0.007 to 0.03). Growth curves (C) for Kelly cells following transfection with various combinations of AKT2 expression vector, empty vector, miR-184 mimics or negative control (NC) oligonucleotides. The highest number of cells was produced by either the AKT2 expression vector alone or in combination with a negative control oligonucleotide. These cells had both endogenous and ectopic AKT2 (Additional File 5). Co-transfection with the AKT2 expression plasmid (lacking the miR-184 target site) and miR-184 mimics yielded a cell number curve that was not statistically different from co-transfection with a negative control oligonucleotide and the empty expression vector. The former cell population had ectopic but not endogenous AKT2 activity, while the latter cell population had endogenous, but no ectopic AKT2. Both of these cell number curves were significantly different from the other curves (p < 0.003). Transfection of miR-184 mimics by themselves or in combination with the empty vector significantly impacted cell growth, as these cells had no ectopic AKT2 and endogenous AKT2 was inhibited.

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