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Figure 1 | Molecular Cancer

Figure 1

From: PI3Kp110-, Src-, FAK-dependent and DOCK2-independent migration and invasion of CXCL13-stimulated prostate cancer cells

Figure 1

Differential expression of PI3K catalytic isoforms and DOCK2 by PCa cell lines. (A) Total cell lysates (60 μg) from RWPE-1, LNCaP, and PC3 cells were resolved by SDS-PAGE and subjected to immunoblotting using antibodies against PI3Kp110α, p110β, p110γ, p110δ, and DOCK2 (Santa Cruz). GAPDH detection served as a loading control. (B) DOCK2 silencing conditions were optimized by transfecting PC3 cells with 1 μg of DOCK2 siRNA duplex incubated cells for 0, 24, 48, and 72 hours. The efficacy of DOCK2 silencing was determined by Western blot analysis.

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