Figure 3From: PI3Kp110-, Src-, FAK-dependent and DOCK2-independent migration and invasion of CXCL13-stimulated prostate cancer cellsPI3K regulation of PCa cell migration and invasion. LNCaP and PC3 cell lines were tested for their ability to invade Matrigel™ Matrix and migrate through an 8.0 μm porous membrane in the presence of CXCL13 and/or wortmannin, PI-103, TGX-221 and AS605240, U73122, or DOCK2 siRNA. Cells that invaded or migrated to the lower surface of the membrane were stained with crystal violet and counted by microscopy at 40× magnification. Percent cell invasion was calculated following manufacturer's instructions (BD Biosciences). Asterisks indicate significant differences (p < 0.05) with CXCL13-treated cells.Back to article page