Figure 5

ERK1/2 Activation by CXCL13-treated PCa cells. (A) RWPE-1, LNCaP, and PC3 cell lines were serum starved, treated with 100 ng/ml CXCL13 for 0, 5, 10, 15 and 30 minutes, and lysed. Cell lysates were subjected to immunoblotting with antibodies against p-ERK1/2. Membranes were stripped and reprobed with antibodies to total ERK1/2. (B) Densitometry analysis was performed using VersaDoc Imaging System (Biorad). Histograms show the differences in ERK1/2 levels between LNCaP and PC3 cells at basal activity and after CXCL13 stimulation. Asterisks indicate significant differences (p < 0.05).