Skip to main content
Figure 4 | Molecular Cancer

Figure 4

From: A retroviral mutagenesis screen identifies Cd74 as a common insertion site in murine B-lymphomas and reveals the existence of a novel IFNγ-inducible Cd74 isoform

Figure 4

Identification of a novel Cd74 isoform. (A) Alignment of the N-terminal part of the ORFs in the canonical (Cd74Wt) and the novel alternative isoform (Cd74Alt), respectively. The two ORFs only differ in the N-terminus and are identical from entry into exon 2, which is illustrated in the figure by a grey arrow. The asterisk marks the methionine subject to codon mutation in Cd74Alt_mut in (B) and the arrowhead marks the site of proteolysis. (B) Coupled transcription/translation reaction in vitro with T7-driven expression of Cd74Wt (lane 1), Cd74Alt (lane 2), and Cd74Alt with the predicted start codon mutated (lane 3, Cd74Alt_mut) in the presence of 35S-labelled methionine.

Back to article page

Molecular Cancer

ISSN: 1476-4598