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Figure 6 | Molecular Cancer

Figure 6

From: A retroviral mutagenesis screen identifies Cd74 as a common insertion site in murine B-lymphomas and reveals the existence of a novel IFNγ-inducible Cd74 isoform

Figure 6

IFNγ-responsiveness of the novel intronic Cd74 promoter. (A) RT-PCR analysis on RNA from NIH 3T3, MPC11, and MC3T3 cells, detecting the canonical transcript initiated in exon 1 (Wt) or the alternative transcript initiated just upstream exon 2 (Alt), respectively, without (-) or with (+) IFNγ-stimulation. The RT-PCRs were performed with 1:10 diluted cDNA as template for detection of the canonical Cd74 transcript and with undiluted cDNA as template for detection of the transcript encoding the novel isoform. Gapdh-specific RT-PCR is included as control. (B) Quantitative PCR analyses on NIH 3T3 cells treated with 25 ng/ml IFNγ for 24 and 48 hours, respectively, prior to harvest of RNA. The signal of the Cd74 transcript was normalized to the expression level of TATA-box binding protein in individual cDNA preparations and fold induction compared to un-treated NIH 3T3 cells was calculated on the basis of three independent experiments.

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Molecular Cancer

ISSN: 1476-4598