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Figure 5 | Molecular Cancer

Figure 5

From: Organ-, inflammation- and cancer specific transcriptional fingerprints of pancreatic and hepatic stellate cells

Figure 5

Quantitative mRNA analysis of Collagen11a1 and its localization in tissues and cultured stellate cells. Quantitative real-time PCR (A) was performed with the Light Cycler Fast Start DNA SYBR Green kit. The organ specific expression of Collagen11a1 is depicted in the last two columns of the graph. Double-staining and ex vivo immunofluorescence analysis is used to evaluate the colocalization (orange) of the typical stellate cell marker α-SMA (red) and Collagen11a1 (green) in activated stellate cells of pancreatic cancer tissues (B, 630×). Tissues of primary pancreatic ductal adenocarcinoma (C, 200×, D, 50×), chronic pancreatitis (E, 200×), liver cirrhosis (F, 200×) and liver metastasis of pancreatic ductal adenocarcinoma (G, 200×, H, 50×) were immunostained with a specific antibody against Collagen11a1. Negative controls are shown as insets. Arrows show the stellate cells/myofibroblasts in the fibrotic parts of the tissues.

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