Figure 1

Effect of cyclopamine on androgen signaling in LNCaP cells. (A) Real time qPCR was used to measure relative expression of KLK3, KLK2, PGC or SHH mRNA in androgen-supplemented (+R1881) or androgen deprived (-R1881) LNCaP or in LNCaP-AI cells (-R1881) in the presence of vehicle (EtOH) or with 5 or 10 μM cyclopamine (Cyc-5, Cyc-10) (also see Additional file 2, Table S2). (B) LNCaP or LNCaP-AI cells were infected with probasin (PRB) or PGC promoter reporter vectors along with a CMV-GFP reference reporter and were cultured in androgen depleted medium with vehicle (EtOH) or with 5 or 10 μM cyclopamine (Cyc-5 or Cyc-10) for 72 hrs. Cell extracts were assayed for luciferase that was normalized by GFP intensity. Bars represent the means of triplicate experiments ± S.E. (* = P < 0.05 compared to vehicle control; ** = P < 0.05 between 5 and 10 μM cyclopamine treatment groups).