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Figure 2 | Molecular Cancer

Figure 2

From: Hedgehog/Gli supports androgen signaling in androgen deprived and androgen independent prostate cancer cells

Figure 2

Smo knockdown affects androgen responsive gene expression in androgen-deprived LNCaP cells. (A) LNCaP cells were transfected with control (Ctrl) siRNA, AR or Smo siRNA and grown in androgen-depleted medium for 72 hrs. RNAs were extracted and assayed by real-time qPCR for expression of AR, Smo, KLK2 or KLK3. Bars represent the means of three experiments ± S.E. (* = P < 0.05 compared to control siRNA). (B) Cells transfected with siRNA were infected with a Gli or Probasin (PRB) FF luciferase reporter lentivirus along with a CMV-GFP lentivirus control reporter and were switched to androgen-depleted medium for 72 hrs. Cell extracts were quantified for luciferase activity that was normalized by GFP intensity. Bars represent the means of triplicate experiments ± S.E. (* = P < 0.05 compared to control siRNA). (C) Western blot shows effects of siRNA on expression of AR protein in cell lysates.

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Molecular Cancer

ISSN: 1476-4598