Figure 3From: Hedgehog/Gli supports androgen signaling in androgen deprived and androgen independent prostate cancer cellsCyclopamine prevents the development of AI prostate cancer cell growth and suppresses the growth of LNCaP-AI cells. (A) Phase contrast photomicrographs (40×) of LNCaP cells cultured for 60 days in androgen depleted medium (CS-FBS) supplemented with vehicle (EtOH) or 5 μM cyclopamine (Cyclo). Cell numbers in cyclopamine are greatly reduced and cells have optically dense, fragmented nuclei. (B) LNCaP-AI cells grown in androgen-depleted medium (CS-FBS) supplemented with vehicle (EtOH) or 5 μM cyclopamine. Cell numbers were counted at various days as indicated. Points represent the means of triplicate cultures ± S.E.Back to article page