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Figure 2 | Molecular Cancer

Figure 2

From: Vascular endothelial growth factor regulates myeloid cell leukemia-1 expression through neuropilin-1-dependent activation of c-MET signaling in human prostate cancer cells

Figure 2

VEGF 165 regulates Mcl-1 expression in PCa cells. (a) VEGF expression in ARCaPM and ARCaPM-C2 cells, as determined by RT-PCR and ELISA (conditioned medium). (b) Effects of recombinant VEGF165 on cell proliferation as determined by MTS assay. Top, ARCaPM cells were seeded in 96-well plates (1 × 103cells/well) for 24 h, and serum-starved overnight. The cells were further incubated with varying concentrations of VEGF165 in serum-free T-medium for 72 h. Bottom, ARCaPM cells were incubated with PBS or VEGF165 (50 ng/ml) in serum-free T-medium for varying times. (c) VEGF165 effects on Mcl-1 expression. ARCaPM cells were treated with VEGF165 at indicated concentrations and times, and Mcl-1 mRNA expression was measured. For immunoblotting, ARCaPM cells were incubated with VEGF165 (10 ng/ml) for 72 h. (d) Effects of VEGF siRNA on Mcl-1 expression. ARCaPM cells were transfected with VEGF siRNA or control siRNA (80 nM) for 72 h.

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