Figure 1
SPDCs repress the growth of B16 tumors when co-inoculated with tumor cells. (A) The B16 tumor cells (5 × 106) were mixed without or with wild type SPDCs (1 × 106), and were injected subcutaneously into normal mice. The tumor volume was monitored every 2 days from the 5th day after the inoculation, by measuring the tumor length (L) and short (S) with a sliding caliper. Tumor volume = L × S2 × 0.51. (B) The tumors were dissected 13 days after the inoculation, and the tumor weight was compared. (C) qRT-PCR analysis. DCs in the spleens of wild type and B16 tumor-bearing mice were sorted by anti-CD11c magnetic beads. The Notch downstream genes were analysed by qRT-PCR, with β-actin as an internal control. Bars, means ± SD, * P < 0.05 (n = 4).