Figure 3

Infiltration of CD4⁺, CD8⁺, CD19⁺ and NK1.1⁺ cells in the tumors and draining lymph nodes of the mice bearing B16 and H22 tumors. The tumor cells were mixed with RBP-J^+/- and RBP-J^-/- SPDCs, and were injected subcutaneously into normal mice. (A) The tumors were dissected on the 17th day after the inoculation, and the single-cell suspensions were prepared for FACS analyses. The number of CD4⁺, CD8⁺, CD19⁺ and NK1.1⁺ cells in 1 g of tumor tissues was calculated based on FACS. (B) The draining lymph nodes were dissected, and the total cell number in the lymph nodes was compared between the RBP-J^-/- and RBP-J^+/- groups. (C) FACS was carried out to compared the number of CD4⁺, CD8⁺, CD19⁺ and NK1.1⁺ cells in the draining lymph nodes of mice bearing melanoma containing the RBP-J deficient SPDCs or the control SPDCs. Bars, means ± SD. n = 4. (D) DNA content analysis of the cells isolated from the draining lymph node of the mice bearing melanoma containing the RBP-J deficient DCs or the control DCs. Pictures represent one set of four independent experiments.