Figure 4
Cell migration assay. (A, B) SPDCs with different genotypes were co-cultured with the B16 tumor antigens, and the expression of CCR7 was analyzed by flow cytometry. The mean fluorescence intensity (MFI) of CCR7 expression was compared between groups. Bars, means ± SD. *P < 0.05. n = 3. (C) SPDCs with different genotypes were stained with CFSE, and were incubated with the B16 tumor antigens. SPDCs (1 × 106) were then injected subcutaneously at the left lower abdomen, and the draining lymph nodes were dissected 12 h later. CFSE positive DCs were detected by FACS, and the number of CFSE positive DCs in the draining lymph nodes was calculated based on FACS. Bars, means ± SD. n = 3.