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Figure 1 | Molecular Cancer

Figure 1

From: 5-ALA mediated photodynamic therapy induces autophagic cell death via AMP-activated protein kinase

Figure 1

ALA-PDT-induced oxidative stress results in mitochondrial dysfunction and cell death. Survival rate of PC12 cells (A) and CL1-0 cells (B) after ALA-PDT. The right panel shows the cell survival rate of PC12 and CL1-0 cells which were incubated with 1 mM ALA for 3 hr, and then irradiated with different light dose. The left panel shows the survival rate of PC12 or CL1-0 cells pre-incubated with different concentrations of N-acetylcysteine (NAC) before ALA-PDT (1 mM ALA, 2 J/cm2 and 12 J/cm2 of light for PC12 and CL1-0 cells, respectively). Cell survival was assessed by Trypan Blue exclusion assay 24 hours after light irradiation. Data were obtained from three independent experiments and are shown as mean ± SD. Significantly different from ALA only; *P < 0.05, **P < 0.01, ***P < 0.001. (C) Mitochondrial membrane potential decreased following ALA-PDT in PC12 cells. JC-1 labeling of the cells treated with ALA only (left panel), right after (middle panel) or 30 minutes post light irradiation (right panel) under the light dose of 2 J/cm2 are shown. The upper panel represents the JC-1 monomer (green) and lower panel represents the JC-1 aggregate (red). (D) Following ALA-PDT (1 mM ALA, 2 J/cm2), PC12 cells lysates were prepared at the indicated times and the ATP contents were measured using an ATP determination kit. The figure is a representative of three independent experiments and the results are shown as mean ± SD. Significantly different from ALA only; *P < 0.05, **P < 0.01.

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