Figure 5
From: 5-ALA mediated photodynamic therapy induces autophagic cell death via AMP-activated protein kinase
AMPK mediates autophagic cell death induced by ALA-PDT. Effects of autophagy inhibitor, 3-MA, on ALA-PDT mediated cell death and caspase activity in PC12 (A) and CL1-0 (B) cells. PC12 and CL1-0 cells were pre-treated with different concentration of 3-MA before ALA-PDT. The light dose for PC12 and CL1-0 cells were 2 and 12 J/cm2, respectively. Cell survival was analyzed 24 hr post ALA-PDT, and the caspase activity was analyzed 4 hr post ALA-PDT. (C) Expression level of LC-3 in PC12 and CL1-0 cells in response to Compound C treatment. Cell lysates were collected at 8 hr post ALA-PDT for LC-3 western blot. β-actin was used as an internal control.