Figure 4
From: Myc interacts with Max and Miz1 to repress C/EBPδ promoter activity and gene expression
Miz1 binds to the C/EBPδ proximal promoter. A. Miz1 binds to the C/EBPδ promoter (170 bp, -140 to +30). Lanes: (1) C/EBPδ 170 bp promoter fragment; (2) Miz1 (5 ng) + C/EBPδ 170 bp promoter fragment; (3) Miz1 (15 ng) + C/EBPδ 170 bp promoter fragment; (4) Miz1 (5 ng). B. Miz1 binds to the C/EBPδ promoter 5' region. Lanes: a. C/EBPδ promoter fragment (-140 to +30), - or + Miz1 (5 ng); b. C/EBPδ promoter fragment (-140 to -10), - or + Miz1; c. C/EBPδ promoter fragment (-140 to -40), - or + Miz1; d. C/EBPδ promoter fragment (-140 to -70), - or + Miz1. C. Miz1 does not bind to C/EBPδ promoter fragments with the -110 to -80 region deleted. Lanes: a. C/EBPδ promoter fragment (-140 to +30), - or + Miz1 (5 ng); e. C/EBPδ promoter fragment (-110 to +30), - or + Miz1; f. C/EBPδ promoter fragment (-80 to +30), - or + Miz1; g. C/EBPδ promoter fragment (-50 to +30), - or + Miz1. Results are representative of 3 EMSA experiments.