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Figure 5 | Molecular Cancer

Figure 5

From: Myc interacts with Max and Miz1 to repress C/EBPδ promoter activity and gene expression

Figure 5

Miz1 binding is localized to the -100 to -70 region of the C/EBPδ proximal promoter (Probe "i"). A. EMSAs were performed without Miz1 (-) or with Miz1 (+) (5 ng) plus the following C/EBPδ proximal promoter fragments: -127 to -100 bp (Probe "h"); -100 to -70 (Probe "i"); or +1 to +30 (Probe "j") - or + Miz1 (5 ng). B. Competition EMSAs: C/EBPδ -100 to -70 proximal promoter fragment (Probe "i") effectively competes for Miz1 binding with the "full length" -140 to +30 C/EBPδ proximal promoter fragment. Miz1 was incubated with the "full length" -140 to +30 C/EBPδ proximal promoter fragment (Probe "a") plus 5×, 10× and 25× molar excess of C/EBPδ proximal promoter fragments: -127 to -100 bp (Probe "h"); -100 to -70 (Probe "i"); or +1 to +30 (Probe "j"). Results are representative of 2 EMSA experiments.

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