RuvBl1 and RuvBl2 repress C/EBPδ promoter activity. A. Exponentially growing HC11 cells were co-transfected with vector control (VC) or increasing amounts (20, 50 100 μg) of FLAG-tagged RuvBl1 (TIP49, Pontin), RuvBl2 (TIP48, Reptin) or RuvBl1/RuvBl2 combined plus the C/EBPδ proximal promoter-luciferase and the Renilla control. Luciferase results were normalized to the Renilla control, the VC values were set as "1" and the RuvBl1 and RuvBl2 transfected cell luciferase results are expressed relative to the VC control. B. Lysates from luciferase assays (A.) were analyzed by Western blot to using an anti-FLAG antibody to assess Myc and Miz1 protein levels. β-actin levels were assessed as a loading control. All luciferase results shown are from 3 independent experiments with duplicates performed in each experiment.