Reversal of BPDE's effect on RAR-β
expression using 5-Aza. (A) ChIP assay. Esophageal cancer TE-3 cells were grown and treated with or without 1 μM BPDE, 10 μM 5-Aza, or the combination of both for 12 h, and the cells were then subjected to ChIP analysis with anti-BPDE antibodies. The immunoprecipitated protein was subjected to Western blotting analysis of DNMT3A expression. Proteins without anti-BPDE antibody immunoprecipitation (input) were used as controls. (B) Northern blotting. Total RNA from TE-3 and TE-12 cells with the same treatment was isolated and subjected to Northern blotting analysis of RAR-β2 expression.