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Figure 5 | Molecular Cancer

Figure 5

From: Benzo[a]pyrene diol epoxide suppresses retinoic acid receptor-β2 expression by recruiting DNA (cytosine-5-)-methyltransferase 3A

Figure 5

Effects of DNMT3A shRNA. (A) Knockdown of DNMT3A expression by DNMT3A shRNA. Esophageal cancer TE-3 cells were grown and transiently transfected with DNMT3A 29 mer shRNA constructs in pRS vector; 48 h later, total cellular protein was extracted and then subjected to Western blotting analysis of DNMT3A expression. (B) RT-PCR analysis of RAR-β2 mRNA expression. Esophageal cancer TE-3 cells were grown and transiently transfected with DNMT3A shRNA-3 vector; 48 h later, the cells were treated with or without 1 μM BPDE for 12 h and RNA was then isolated from the cells and subjected to RT-PCR analysis.

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