Figure 6From: ISG20L1 is a p53 family target gene that modulates genotoxic stress-induced autophagyISG20L1 decreases cell survival that is partially rescued in ATG5-/- MEFs. (a) HCT116 cells were transfected with pCEP4 vector control or ISG20L1, selected in hygromycin B, and the number of colonies formed after selection were counted using BioRad Quantity One software, shown in the lower panel. Duplicates of each transfection are shown. Similar results were observed in cultures of RKO and H1299 cells (data not shown). Results represent the mean and standard error. (b) ATG5+/+ and ATG-/- MEFs were transfected with vector control (pCEP4) and ISG20L1; selected in hygromycin B; and the number of colonies formed after selection were counted using BioRad Quantity One software. Representative pictures are shown (upper panels) for each plasmid in both autophagy proficient (ATG5+/+) and deficient (ATG-/-) MEFs. Colony number quantified in the lower panel represent mean and standard error of each transfection for both ATG5+/+ and ATG-/- MEFs performed in triplicate.Back to article page