Figure 5
From: Molecular mechanism of chemoresistance by miR-215 in osteosarcoma and colon cancer cells
miR-215 inhibits cell proliferation and triggers cell cycle G2-arrest by down-regulation of DTL and subsequent increased p53 and p21 in HCT 116 (wt-p53) cells. (A) The DTL protein was down-regulated in colon cancer HCT 116 (wt-p53) cells transfected with miR-215 (100 nM) analyzed by Western immunoblot analysis, DTL specific siRNA (100 nM) was used as the positive control. (B) Suppression of DTL by DTL specific siRNA resulted in growth inhibition in HCT 116 (wt-p53) cells. Numbers are indicated as mean ± SD. (C) Down-regulation of DTL triggered cell cycle G2-arrest in HCT 116 (wt-p53) cells transfected with DTL specific siRNA. This experiment was repeated two separate times, and similar results were obtained. The representative flow cytometry pattern was shown.