Figure 4
From: NF-κB/STAT3/PI3K signaling crosstalk in iMycEμ B lymphoma
STAT3 inhibition reduces growth, leads to apoptosis and downregulates NF-κB and Myc activity in iMycEμ-1 cells. (A) Dose-dependent suppression of proliferation (MTS/PMS) after culture with the STAT3 inhibitor WHI P-131 (WHI) at various concentrations, as indicated. Data were normalized to DMSO treatment controls, and error bars represent the standard deviation for a representative experiment performed in triplicate. (B) Agarose gel showing DNA fragmentation after treatment with WHI. (C, D and E) EMSA revealing reduced STAT3 (C), NF-κB (D) and Myc (E) DNA-binding, respectively, after WHI treatment. (F) Western blot showing a decrease in the level of Myc protein after STAT3 inhibition. β-actin was used as a loading control. All WHI incubations were for 24 hours.