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Figure 1 | Molecular Cancer

Figure 1

From: miR-29b defines the pro-/anti-proliferative effects of S100A7 in breast cancer

Figure 1

S100A7 differentially regulates miR-29b transcription and NF-κB activation in MDA-MB-231 and MCF7 cells. (A) GFE analysis was performed on the top up/down-regulated genes of 231-S100A7 and MCF7-S100A7 cells. Genes up/down-regulated > 2 folds in 231-S100A7 and those up/down-regulated > 2.5 folds (similar amounts of genes) in MCF7-S100A7 were filtered from microarray data and used as training sets for separate analysis. Training sets from microarray data are indicated by heat maps, and percentage of miR-29b targets are indicated with pie charts with significance of enrichment analysis. (B) mature miR-29b was measured by qRT-PCR in the given cell lines, with RNU6B as internal control. Values are not comparable between MDA-MB-231 and MCF7 cells. (C) primary mir-29b-1 and mir-29b-2 transcript levels were detected by qRT-PCR using specific primers. 18S rRNA was used as internal control. (D) total cell lysates were subjected to κB-site-DNA-bait ELISA assay to detect total active NF-κB dimer level. Values are not comparable between MDA-MB-231 and MCF7 cells. (E) cells transfected with NF-κB reporter plasmids were harvested after 48 h, luciferase activity was analyzed in cell lysates. (F) activated p-p65 subunit of NF-κB in different cells were observed with confocal microscopy. Nuclei were stained with DAPI. (G) nuclear and cytoplasmic portions of cells were extracted and activated p65 NF-κB were detected with κB-site-DNA-bait ELISA. Data show the relative percentage of active NF-κB in the two portions. (*p < 0.05, **p < 0.01).

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