Figure 2

S100A7 differentially regulates miR-29b transcription in MDA-MB-231 and MCF7 cells. (A) MCF7 cells were treated with 5nM QNZ for 24 h before they were harvested for qRT-PCR analysis. A well-known NF-κB driven gene GM-CSF served as the positive control for effect of QNZ inhibition. (B) κB binding sites at the proximal of mir-29b-1 gene transcriptional start site. Two sets of primers, κB-1 and κB-2, were designed to detect three κB binding sites in ChIP. (C) indirect inhibition of mir-29b-2 transcription by NF-κB and YY1. (D) ChIP assay of NF-κB p65 subunit binding to mir-29b-1 promoter region in 231-V and 231-S cells. (E) ChIP assay of NF-κB p65 subunit binding to mir-29b-1 promoter region in MCF7-V (M-V) and MCF7-S (M-S). (F) qRT-PCR quantification of YY1 level in different cells. (*p < 0.05, **p < 0.01, N.S.: non-significant).