Skip to main content
Figure 5 | Molecular Cancer

Figure 5

From: SPOCK1 as a potential cancer prognostic marker promotes the proliferation and metastasis of gallbladder cancer cells by activating the PI3K/AKT pathway

Figure 5

SPOCK1 promotes tumor invasion and metastasis in vitro by inducing EMT. (A) Wound closure was delayed in Lv-shSPOCK1-transduced cells compared with that in CTRL and Lv-shNC cells at 48 h in both GBC-SD and NOZ cells. Overexpression of SPOCK1 in SGC-996 cells had the opposite effects (*P < 0.05, **P < 0.01, and ***P < 0.001). (B) Matrigel invasion assay of CTRL, Lv-shNC, Lv-shSPOCK1, MOCK, and SPOCK1 transfectants cells. The number of invaded cells was calculated and is depicted in the bar chart. (*P < 0.05, **P < 0.01, and ***P < 0.001). (C and D) The protein expression of Snail, vimentin, N-cadherin and E-cadherin in the indicated cells was examined by western blotting. The protein expression of vementin and E-cadherin was examined by immunofluorescence analysis. The red signal represents staining for E-cadherin or vimentin. Nuclei were counterstained with DAPI.

Back to article page

Molecular Cancer

ISSN: 1476-4598