Skip to main content
Figure 7 | Molecular Cancer

Figure 7

From: SPOCK1 as a potential cancer prognostic marker promotes the proliferation and metastasis of gallbladder cancer cells by activating the PI3K/AKT pathway

Figure 7

SPOCK1 exerts an anti-apoptotic effect via the PI3K/Akt pathway. (A) Apoptosis was determined by flow cytometry. Cells stained with annexin-V-APC were considered as apoptotic. The apoptotic index was defined as the percentage of apoptotic cells. (B) Apoptotic changes in the nuclear morphology of GBC-SD and NOZ cells as indicated by Hoechst 44322 staining (blue). The apoptotic index, defined as the percentage of apoptotic cells, was calculated and is summarized in the bar chart (*P < 0.05, **P < 0.01, and ***P < 0.001). (C) The levels of phosphorylated PI3K (Tyr607), total PI3K, phosphorylated Akt (Ser473), total Akt, Bax, Bcl-2, caspase-9, caspase-3, and PARP were detected in CTRL, Lv-shNC, and Lv-shSPOCK1 cells by western blot analysis. GADPH was used as the loading control.

Back to article page

Molecular Cancer

ISSN: 1476-4598