Effect of phenytoin on invasion, proliferation, apoptosis and angiogenesis. (A) Tumour sections stained with H&E showing (i) mammary fat pad and (ii) skeletal muscle invasion. Arrows, infiltration of tumour cells (T) into fibroadipose tissue (F) or skeletal muscle fibres (M). (B) Tumour stained with anti-MMP9 (red) and DAPI (blue). (C) Tumour stained with anti-Ki67 (red) and DAPI (blue). (D) Tumour stained with anti-activated caspase-3 (red) and DAPI (blue). (E) Blood vessels stained with anti-CD31 (red) and DAPI (blue). (F) MMP9+ cells/mm2 (n = 40) (G) Ki67+ nuclei/mm2 (n = 40). (H) Activated caspase-3+ cells/mm2 (n = 40). (I) CD31+ blood vessels/mm2 (n = 40). Data are mean + SEM; **P < 0.01; ***P < 0.001. Scale bars, 100 μm.