Effect of phenytoin on metastasis. (A) Bioluminescent images of metastases in control and phenytoin-treated mice. (B) Bioluminescence measured from the indicated anatomical sites (n ≥ 10). (C) Bioluminescence measured ex vivo from the liver, lungs and spleen (n = 11). Liver (D), lungs (E), and spleen (F) stained with anti-GFP (green) and DAPI (blue). (G,H,I) GFP+ cells/mm2 at each site (n ≥ 249). Data are mean + SEM; *P < 0.05; ***P < 0.001. For (B) and (C), P < 0.01 between control and phenytoin (two-way ANOVA). Scale bar, 100 μm.