Figure 3

iMSC#3 secretes PGE ₂ , which can be inhibited by Indomethacin. iMSC#3 cells were grown to confluency, and growth medium was changed from MEMα to RPMI with or without Indomethacin as indicated. After 24 h, primary BCP-ALL cells were added as indicated and cocultures incubated for an additional 24 h. Supernatants were subsequently subjected to analysis of prostaglandin E₂ levels by EIA as described in Materials and Methods. The results are presented as scatter plots with horizontal bars representing median values. *P < 0.05 by Wilcoxon matched pairs signed rank test.