Syndecan-2 expression correlates with metastatic status in patient. (A) Normal breast tissues (Normal) and breast cancer patient tissues (Cancer) from tissue microarrays were stained for syndecan-2 or caveolin-2 on serial sections. Magnified images are shown in lower panels, bars = 100 μm. (B) Intensity per area for syndecan-2 or caveolin-2 staining was quantified for each core in tissue microarray to compare normal breast tissue and patient breast tissue of different tumour grades, n = 161 for syndecan-2 and n = 165 for caveolin-2. Bar graph was plotted in ln scale. (C) Breast tissue from patients diagnosed with Grade II carcinoma and metastatic lymph node tissue from the same patient were stained for syndecan-2 and caveolin-2. Higher magnification images are shown in lower panels, bars = 100 μm. (D) Quantification data of intensity per area of syndecan-2 or caveolin-2 staining from (C), n = 26 for syndecan-2 cases and n = 28 for caveolin-2 cases. Bar graph was plotted in ln scale. Error bars = s.e.m. *p < 0.05, **p < 0.01, ***p < 0.001, n.s.: not significant, tested by one-way ANOVA with Bonferroni post-hoc test. (E) Levels of syndecan-2 and caveolin-2 staining from serial sections of the 47 triple negative breast carcinoma cases were significantly correlated. r: Spearman’s correlation coefficient = 0.328, p = 0.024.