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Figure 5 | Molecular Cancer

Figure 5

From: OTUB1 de-ubiquitinating enzyme promotes prostate cancer cell invasion in vitro and tumorigenesis in vivo

Figure 5

PC3 cells xenografts expressing shOTUB1 show decreased tumorigenesis and metastatic activity. (A) PC3 cells expressing a short harpin cDNA targeting OTUB1 or a scramble control were used to measure anchorage independent growth in soft agar. (B) PC3-shOTUB1 or PC3-shControl cells were implanted subcutaneously (n = 4 mice per group) and tumor growth was measured weekly. (C) Immunohistochemical analysis of the expression of OTUB1 and Ki-67 in the xenograft tumors. Three random areas per mouse and tumor were counted for the expression of both proteins and the results are shown as percentage of positive cells. (D) PC3-shOTUB1 or PC3-shControl cells were implanted into the prostate of nude mice (n = 4). After 8 weeks, orthotopic tumors were surgically removed and their volumes analyzed. (E) Number of lymph node metastatic foci per mouse. *indicates statistically significant (p < 0.05) changes between shOTUB1 cells and shControl cells.

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