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Figure 1 | Molecular Cancer

Figure 1

From: Targeting TR4 nuclear receptor suppresses prostate cancer invasion via reduction of infiltrating macrophages with alteration of the TIMP-1/MMP2/MMP9 signals

Figure 1

Knocking down TR4 reduces macrophage recruitment to PCa cells in co-culture system using Transwell assays. A. The illustration of macrophage recruitment (migration) and PCa invasion (invasion) in vitro model. The PCa cells were placed on the upper chamber and THP-1 cells in the lower chamber, co-cultured for 24 hours and then four kinds of conditioned media (CM) were collected: PCa scr/THP-1 scr, PCa siTR4/THP-1 scr, PCa scr/THP-1 siTR4, PCa siTR4/THP-1 siTR4. The CMs were diluted with 10% FBS RPMI media at 1:1 ratio, then put into the lower chamber of other transwell plates for macrophage migration assays and PCa cells invasion assays. B. The manipulation of TR4 in PCa cells and THP-1 cells. Western blotting to show the knock-down efficiency of TR4 in C4-2 and CWR22Rv-1 PCa cell lines and THP-1 macrophage cell line. C-E. Macrophage recruitment under different CMs. Upper are CM from C4-2/THP-1 cells and lower are from CWR22Rv1/THP-1 cells. Knock-down of TR4 in both PCa and THP-1 cell lines is shown in the left panels, knock-down of TR4 in PCa cells alone shown as D and knock-down of TR4 in THP-1 cells alone shown in as E. Note: * P < 0.05; ** P < 0.01; ns P > 0.05.

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Molecular Cancer

ISSN: 1476-4598