Figure 5

Knockdown of tumor derived MMP13 leads to decreased tumor metastasis to the liver. (a) Representative liver cross sections stained with Haematoxylin & Eosin of wildtype mice injected with control or MMP13 knockdown MC38 cell lines (MSh13 pooled, MSh13-1, and MSh13-2). Dashed black lines denote tumors. Quantification of metastatic tumor burden measured by (b) liver weight, (c) tumor burden as a percent liver weight to total body weight, (d) metastatic seeding by quantification of tumor number and (e) percentage of tumor area to total liver area in normal and steatotic livers (n = 5 per group). Statistical analysis was performed using GraphPad Prism software. Data was analyzed by using one-way ANOVA followed by Newman-Keuls Multiple Comparison Test. P values are represented by stars where: * ≤ .05, ** ≤ .01, and *** ≤ .001 when compared to mice injected with non-silencing control cell lines.