Evidence that the RUNX1-miRNA core mechanism is conserved between human and mouse. (A) Stable ectopic expression of RUNX1-MTG8 in 32D myeloid cells (here shown a representative 32DRUNX1-MTG8 clone) leads to downregulation of both miR-221 and miR-223 relative to a 32DEV control clone. (B) Consistently, CD117 cytofluorimetric analysis shows a significantly (p < 0.05) higher number of KIT-positive cells in 32DRUNX1-MTG8 relative to 32DEV. (C) 32DRUNX1-MTG8 display block of granulocytic differentiation in response to G-CSF, as shown by both CD11b cytofluorimetric analysis (left) and Giemsa staining of cytospin preparations (middle), as well as increased proliferation (right) relative to 32DEV. Shown here one representative clone for each construct.