Figure 3
From: SOX4 interacts with EZH2 and HDAC3 to suppress microRNA-31 in invasive esophageal cancer cells
miR-31 is epigenetically repressed in invasive esophageal cancer cells. TE8 and FLO1 cells were treated at indicated concentrations with Polycomb/EZH2 inhibitor (DZNep), Histone deacetylase inhibitor (SAHA) and DNA methyl-transferase inhibitors (AZA). (A) DZNep treatment for 72 hours dose-dependently inhibits EZH2 and EZH1 in FLO1 and TE8 cells as shown by western blotting. (B) qRT-PCR analysis of miR-31 expression in TE8 and FLO1 cells treated with DZNep for 72 hours. (C) SAHA treatment for 48 hours inhibits HDAC3, EZH2 and EZH1 in TE8 and FLO1 cells in a dose-dependent manner. (D) qRT-PCR analysis of miR-31 expression in TE8 and FLO1 cells treated with SAHA for 48 hours. (E-F) qRT-PCR analysis of miR-31 expression in TE8 and FLO1 cells treated with 5-aza-deoxycytidine (AZA) for 72 hours. miR-31 expression was normalized to RNU6. Results are means ± SD from at least three biological replicates, ANOVA was used for statistical analysis.