Downstream targets of KLF4 in T-ALL. (a) Scatter plots of Dox-treated versus Dox-untreated TRE-KLF4 Jurkat transcriptomes. (b) Unsupervised hierarchical cluster analysis of expression levels of 32 genes that are important for apoptosis, metastasis, T cell, or non-T cell lineages in Dox-treated and Dox-untreated TRE-KLF4 Jurkat cells (red, increased expression; green, decreased expression). (c) Quantitative RT-PCR analysis of selected gene expression in KLF4 overexpressing Jurkat cells. Data are represented as the mean +/- SEM. (d) Western blot detection of T cell specific transcription factors in Jurkat cells 48 hours after KLF4 overexpression. (e) KLF4 binds to the promoters of NOTCH1, CXCR4, and BCL2 through regions containing conserved KLF4 consensus sequences. Chromatin immunoprecipitates were performed on cross-linked fragmented DNAs prepared from Jurkat cells that forcefully expressed KLF4. Eluted DNAs were then analyzed by qPCR performed with primers flanking putative KLF4-binding sites. The amount of DNA amplified from immunoprecipitated DNAs was normalized to that amplified from input DNA. Data are represented as the mean +/- SEM.