Figure 5
Analysis of ID protein expression levels in primary CLL. (A) Western blotting analysis of ID2 and ID3 expression was performed in two separate experiments depicted in the two panels shown. CLL10 and CLL11 samples were included in both experiments to monitor internal consistency. Immunoblots were re-probed with anti-GAPDH antibody as a control for protein loading. The original western blot images that were used to compile Figure 5A are shown in Additional file 8: Figure S3. (B) Protein bands were quantified by densitometric scanning and normalized to the GAPDH loading control and, for each ID protein, expressed as fold-change (relative expression level) relative to the CLL sample with the lowest expression level on the left blot (CLL08 for ID2 and CLL17 for ID3). Densitometric quantification of the western blotting data is shown in Additional file 9: Figure S4. CLL samples are shown rank-ordered by increasing levels of ID3 expression. In vitro IC50 values were determined following 72 hrs of treatment for each CLL sample; ND: not determined.