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Figure 9 | Molecular Cancer

Figure 9

From: ID helix-loop-helix proteins as determinants of cell survival in B-cell chronic lymphocytic leukemia cells in vitro

Figure 9

ID2 and ID3 protein expression patterns in response to in vitro drug treatment. CLL cells from six patients were cultured in the presence of varying concentrations of fludarabine, chlorambucil and ethacrynic acid for 24 hrs. The concentration of drugs were chosen for each CLL, based on IC50 values (see Figure 5B) such that a significant fraction of viable cells remained after the 24 hrs drug treatment period. At the indicated time points, ID2, ID3 and GAPDH (control) levels were analysed by western blotting. The bands were quantified by densitometric scanning and normalized to the intensity of the loading control (GAPDH). The fold-change in expression, relative to the untreated control was determined and, after log2-transformation was displayed as a heat map as shown. For each drug, CLL samples are shown rank-ordered by decreasing IC50 value (decreasing drug resistance).

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